Children |
| 1. PORTUGAL Affonso Prince Of, b. 24 Feb 1526, , Almeria, Almeria, Portugal d. 1526 |
| 2. PORTUGAL Maria Princess Of, b. 15 Oct 1527, , Coimbra, Coimbra, Portugal d. 12 Jul 1545, , Valladolid, Valladolid, Spain (Age 17 years) |
| 3. PORTUGAL Izabel Princess Of, b. 28 Apr 1529, , Lisboa, Lisboa, Portugal d. 1530 (Age 0 years) |
| 4. PORTUGAL Brites Princess Of, b. 15 Feb 1530, , Lisboa, Lisboa, Portugal d. 1530 |
| 5. PORTUGAL Manuel Prince Of, b. 1 Nov 1531, , Alvito, Beja, Portugal d. 14 Apr 1537 (Age 5 years) |
| 6. PORTUGAL Filipe Prince Of, b. 25 May 1533, , Evora, Evora, Portugal d. 29 Apr 1539 (Age 5 years) |
| 7. PORTUGAL Diniz Prince Of, b. 26 Apr 1535, , Evora, Evora, Portugal d. 1 Jan 1539 (Age 3 years) |
| 8. PORTUGAL AND THE ALGARVES Jodao Prince Of, b. 3 Jun 1537, , Evora, Evora, Portugal d. 2 Jan 1554, , Lisboa, Lisboa, Portugal (Age 16 years) |
| 9. PORTUGAL Antonio Prince Of, b. 9 Mar 1539, , Lisboa, Lisboa, Portugal d. 20 Jan 1540 (Age 0 years) |
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